How to acclimatize the roe

The following is a description of the ideal acclimatization process, recommended for use immediately after your batch arrives at your facility. Following these instructions as closely as possible will help to obtain the best possible performance in the incubation and feeding stages.

The complete process can be divided into the following steps:

- Exterior and interior observation: once the transport agent leaves the boxes in your facility, you should ensure that the number of boxes corresponds to the delivery note and make sure there is no external damage to the boxes.

The boxes should then be moved to a cool place and protected from strong light. If possible, acclimatization and incubation through to the hatching should take place in the same spot.

Upon opening the boxes, remove the ice tray, take the other trays out one by one and place them on a surface so that they are all in clear view. Check that the roe has the correct appearance.

- Obtaining details: It is worth noting down the points detailed below:
  • % of melted ice: check that there is still unmelted ice on the tray. This will mean that the humidity and temperature have been correct throughout transport.

  • Temperature of the roe: measure the temperature by placing an adapted thermometer among the roe. Take several readings in various trays and remember that the temperature can vary according to how close to the ice each tray was. If necessary group trays together for acclimatization according to their temperature.

  • Incubation water temperature: measure this temperature with the same thermometer.

  • Calculate temperature difference: the water T minus the roe T ( or vice versa if the water at your facility is colder than the roe)
  • - Acclimatizing: any cooling or heating to a live organism renders it vulnerable to changes which may affect it unbeknownst to us, causing death or deformity in the long term. Therefore, remembering that a change in temperature represents a danger to the life of the embryos the faster the change the greater the danger we will slowly bring the temperature of the roe to the temperature of the incubating water. A safe value for the change in temperature would be 1 degree centigrade every 20 minutes.

    Here we have a description of a possible method of acclimatizing, based on the following example: temperature difference = 5C (water at 10C roe at 5C). Recommended equalizing time = 5 x 20 = 100 minutes.
  • In a garden watering can or similar utensil that can be used to dose the water with a fine spray and create a drip effect, cool water from your installation to a recommended temperature of +1C over the T of the roe, in this case 6C. (you can use the left over ice from the boxes if this is enough to cool the water)

  • Sprinkle water evenly over all the trays and wait for approximately 10 minutes.

  • Repeat this operation with the water at 7C now, distribute it over the trays and wait a further 10 minutes.

  • Use the thermometer to check that the roe in different trays is acclimatizing as desired.

  • Repeat this operation as many times as necessary to balance the temperature of the water and the roe.

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